On Wed, 29 May 2024 17:20:02 +0000, darius wrote:

Barszcz Sosnowskiego - najskuteczniejszym lekiem na raka medycyny
tradycyjnej

https://www.penera.pl/upload/images/2015/07/

barszcz_sosnowskiego_od_amino_2015-07-06_16-23-28.jpg

Pradziadek był znachorem w Małopolsce, z babcią zbieram różne rośliny na
łąkach,
babcia je suszyła i przygotowywała napary, maści, na swoje potrzeby

A tutaj szczegóły i mechanizm antyrakowej terapii, opartej na produktach pochodnych kumaryny, zawaertych Barszczu Sosnowskiego

The search for other more effective methods of control led to a more
detailed study of the chemical composition of Sosnowsky hogweed. It was
found that skin lesions are caused by biologically active compounds - coumarins. The presence of coumarins was detected in the roots,
vegetative organs,
fruits [3, 4, 5]. Furanocoumarins, which are activated by
ultraviolet solar radiation, are especially

https://www.researchgate.net/publication/

347953119_Sosnowsky_hogweed_new_ways_to_use

dangerous. Coumarins, on the other hand, are valuable biologically
active substances. Representatives of coumarins are used in
pharmacology, medicine, cosmetology, and in plant protection methods [5,
6].
They exhibit various properties and types of activity: photosensitizing, sun-protecting [7], antitumor [8–
10], antioxidant and many others [11, 12].

2. Materials and methods Coumarins are a number of natural compounds
based on benzo-α-pyrone.
Coumarins are unsaturated lactones of cis-ortho-hydroxycinnamic acid. In
free form, they are colorless or yellowish crystals, readily soluble in organic solvents and oils. This property formed the basis for the extraction method using ethanol of 95% concentration.
When heated to one hundred degrees Celsius, coumarins are capable of sublimation and crystallize in the form of needle crystals. This
property was used in experiments using the sublimation method with
magnesium oxide. Most coumarins have absorption spectra in the region of 210-270 nm and 290-350 nm. Coumarins dissolve well when heated in
aqueous solutions of alkalis with the formation of salts of
hydroxycinnamic acid. When a small amount of acid is added, coumarins are regenerated and precipitate. This ability of coumarins formed the basis of a qualitative reaction, the so-called "lactone
test", and made it possible to obtain a pure product.
3. The study of the content of coumarins In the biotechnology laboratory
of Yaroslav-the-Wise Novgorod State University in 2019-2020, work was
carried out to isolate coumarins from the dry mass of leaves of Sosnovsky hogweed. Hogweed samples were prepared in June 2019 in
compliance with safety regulations in two regions with different
climatic conditions: in the Valdai district of the Novgorod
region and in the Komi Republic in the vicinity of the city of
Inta. The leaves were dried at room temperature without
sunlight. In further experiments, dry mass, crushed in a porcelain
mortar, was used.
Extraction was performed with ethanol of 95% concentration for four
hours in a Soxhlet apparatus.
The lactone test confirmed the presence of coumarins in the extract. The coumarin content in the extract was determined using a photoelectrocolorimeter at a wavelength of 360 nm. The comparison
solution is ethyl alcohol of 95% concentration. The experimental results
are shown in table 1.
The second method for the extraction of coumarins was the sublimation method with magnesium oxide. The crushed leaves of Sosnowsky hogweed
were mixed with magnesium oxide in a ratio of 1:1.
Heated at a temperature of 100 degrees Celsius for 25 minutes. A round
bottom flask filled with ice was used to precipitate crystals. The mass
of the obtained crystals was determined. Coumarins were isolated from crystals by adding a ten percent alcohol solution of KOH. They were
heated in a water bath for five minutes. Then a solution of hydrochloric acid of 10%
concentration was added. Coumarins precipitated. The precipitate was filtered off and weighed. In this case, coumarins were obtained without impurities of other substances. The results are shown in table 1.

affected the result of the experiment. Therefore, the result was higher
than with sublimation. Sublimation method with magnesium oxide can be considered a better method for the extraction of coumarins. The results
of this method are in good agreement with the results of other
researchers [5]. The disadvantage of this method is the small mass of
the sample subjected to sublimation.

As a result of the experiments, it turned out that the content of
coumarins depends on the growing conditions of Sosnowsky hogweed.
Hogweed, harvested in the Komi Republic near the city of Inta, has a
greater concentration of coumarins than hogweed growing in
Borovichi district of the Novgorod region. Although the plant in the northern regions is much smaller and forms in a short growing season.
And also does not pose a serious danger to people and animals. Based on
the studies conducted, it can be concluded that for the isolation of coumarins, Sosnowsky hogweed,
growing in the Komi Republic,
is the preferred sample.
4. Conclusion The experiments performed indicate that Sosnowsky
hogweed can be
considered not only as an
aggressive weed that has captured numerous territories. Hogweed can be harvested in ecologically safe areas and used as a valuable raw
material for the production of coumarins. Coumarins isolated from Sosnowsky hogweed are planned to be used in further experiments as plant growth stimulants, as part of fungicides and other plant protection preparations

[8]Pattanayaka S P, Bosea P, Sunitab P, Usman M S M and Lapenna A 2018 Bergapten inhibits
liver carcinogenesis by modulating LXR/PI3K/Akt and IDOL/LDLR pathways
Biomedicine & Pharmacotherapi 108 297–308
[9] Emami S and Dadashpour S 2015 Current developments of coumarin-based anti-cancer agents
inmedicinal chemistry Eur. J. Med.Chem. 102 611–30
[10] Dandriyal J et al. 2016 Recent developments of substituted
coumarin derivatives as anticancer
agents Eur. J. Med.Chem. 119 141–68
[11] Chena C, Xua Q, Daia M and Liu X 2019 Bergapten
suppresses RANKL-induced
osteoclastogenesis and JNKsignaling pathways Biophysical Research
Communications 509 329–3

Development of a Liposomal form of Furanocoumarins and Evaluation of its Antitumor Efficacy Article

Oct 2022

Oleg KulikovValentin P. Ageev

Vasilisa ShlyapkinaN. A. Pyataev

View Content of Coumarins in Various Organs of Sosnovsky’s Hogweed (Heracleum Sosnowski Mandena)
Article Full-text available

Sep 2021

L V Andreeva

View

https://www.researchgate.net/publication/

347953119_Sosnowsky_hogweed_new_ways_to_use

Abstract A method for obtaining a liposomal form of furanocoumarins from Sosnowsky’s hogweed (SH) is proposed. Furanocoumarins were isolated from
SH juice using column chromatography. HPLC analysis found that SH juice contains 5-methoxypsoralen (0.04 g/L) and 8-methoxypsoralen (1.35 g/L). Liposomal vesicles with furanocoumarins were obtained by hydration of a
lipid film containing them. The average size of the liposomal vesicles
was 219 ± 23 nm. The resulting liposomes produced an antitumor effect
when administered intravenously to rats with Walker 256 carcinoma
followed by UV irradiation of the tumor node. The antitumor effect of
the liposomes consisted of the photosensitizing effect of the
furanocoumarins and damage to the tumor by UV radiation. The antitumor
action of the furanocoumarins in the liposomal form led to complete
remission of the tumor node in two of seven rats observed for 90 d

it is banned in all Europe !



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