Fish: some fish (a goby) have median lifespans of 60 days, others like guppies, 2 years; A fish listed online is .55 inch (1.4 cm), Danionella, “When the genus was first described, these fish were classified as the smallest adult vertebrates to inhabit
freshwater”; Other fish in the .75 inch to 1 inch size are described at
https://www.myaquariumclub.com/nano-fish-for-small-aquariums-5134.html; Some fish may have greater cognitive ability than some other fish, “Dwarf puffers are one of the few
species of fish that interact with their environment and owner. They will happily greet you as you approach the tank, and can even learn to be hand fed.”; another consideration for mass screening of longevity molecules and longevity genetics and
wellness molecules and wellness genetics at vertebrates is absence of aggression; it is possible that at a communal growth tank frequent fish-fish interaction of a nonpleasant nature could affect physiology, actual physical integrity, and even the
effects of stress on longevity;
Mass screening the fish: at 1 second per fish, a 3d printed 100 fish hydrosorter processes 1 million vertebrate fish for longevity drug efficacy at 10,000 seconds or 167 minutes (2.8 hours); A different engineering version: noting tube lengths, even with
green light causing many fish to prefer forward travel, at 10 seconds per fish, and a 1000 fish (32 times 32 grid) hydrosorter process it is also 2.8 hours to characterize and label 1 million fish; 117 days screens 1 billion fish.
The hydrosorter could be 3d printed so a fresh, absent algae coating, predictable fluid flow hydrosorter is available anytime. One Kg of 3d printing filament is $29; If the hydrosorter has a mass of 1 Kg or likely much less, then a 3d printed version is $
29 That makes CAD development of greater and greater efficacy hydrosorters more rapid, and causes the most complex part to be $29, disposable, and like PLA (polylactic acid), physiologically compatible and harmless.
At the growth tank, fish can be taught light stimulated fish behaviors, green could mean swim towards something, possibly a food source that will dispense food in 2-7 seconds; Blue light could teach the fish to be stationary in the water, possibly
facilitating imaging, Aqua light could be linked to the learning of a 3-7 sequence action the fish could perform for the cameras at the hydrosorter, providing data on cognitive effects at progressive living, White light could teach fish to expect change
without stress; perhaps a mini version of the hydrosorter at the growth tank, only without the cameras, lasers, and other imagers;
Cognitive or cognitive like-distribution shifts, could be measured at the hydrosorter, with accuracy of the aqua light learned sequence, and with efficacious cameras and software, inter-motion time, kind of like velocity of recall; To keep fish moving
through the 1000 channel hydrosorter there could be green light stimulated fish preference for forward travel,
To label individual fish for further study, GFP or another photoshiftable color, then a laser, absent effect on actual fish tissue, barcodes the fish with photochemical modification of just the GFP at 1 million fish labelled each 2.8 hours, also, all the
fish that go through the hydrosorter, notably the labelled fish, can be reused from the after hydrosorter tank, to multiplex treatments; Also, fish the software notes to be of particular interest, like the youngest phenotype fish out of each million, or
if the fish seem euphoric, unusually physically active, or displays mating behaviors at the camera area of the hydrosorter then those effects, potentially beneficial at mammals, can be linked to the longevity chemicals and genes being screened;
There are numerous ways to administer longevity drugs to fish, the hydrosorter could dispense a couple drugged food pellets at a particular area of the hydrosorter, where the mechanism at the hydrosorter could utilize something like n=40 for each
different longevity drug, to administer 25,000 different longevity drugs in 2.8 hours, if the fish get 3 hydrosorter administered doses, separated at 24 hours each, then a full hydrosorter imaging, that is 11.2 hours spread over 96 hours at an automatic
system; I once had a motorized automatic fishfeeder that was about $29.99; Another possible way to administer drugs to fish is to have something like an inkjet printer spray them with a gelcoat, another way is to have the hydrosorter have the fish
breathe drugged water through their gills for a minute at one area of the hydrosorter;
Fish vertebrate research supporting well and longevized human babies: one benefit of using vertebrates like fish as longevity drug development and effectiveness verifiers is that some fish are livebearers of young, this provides an opportunity to see if
there are any beneficial new drug distribution shifting effects of well progeny; it is possible some longevity drugs as well as genes actually heighten fetal and baby wellness, scanning a million fish in 2.8 hours is one way to find drugs that can then
be tested on mammals like mice to find out if fetal and baby well being are increased at mammals as well.
The hydrosorter could be a little like the top view of the internal crenellations of cardboard at about 14 mm width, with sensors tht can see through the 3d printed polymer or a transparent plastic plate on top of the hydrosorter, so one or more cameras
can image the 1000 simultaneous fish for software processing; Notably things like THz imaging of the fish could work through the 3d printed polymer and the software could record things like organ size and skeletal density, and gill respiration rate, and
heartrate, Also, light cameras placed at CAD optimized ports at the 3d printed hydrosorter are also possible, and general soft illumination provided through the 3D printed volume with external lighting;
With positron emission tomography and radiolabelled physiochemicals like like radiolabelled longevity drugs that effect neurons, radiolabelled neurotransmitters, other tissues and molecules, as well as concentration and multiple measurements with time
activity at the physiological tissues with the least vascularization, Then variations or shifts from the longevity drug molecule compared with untreated fish’ distribution of tissue activities and neural function can be gathered as data at a computer
and mapped; it is also possible that noting the 25,000 groups of n=40 fish (1 million) screened each 2.8 hours, that a variety of longevity drug localization effects will be found, and finding out which chemical concentrates where has longevizing and
wellness value.
One use of the unique fish ID produced at something like a laser on GFP is the production of a library of frozen fish, possibly with detectable mRNA activity or things having other technology and drug development value, live clonability from the frozen
fish, also detailed study of the 20-100 most efficacious longevity drugs’ per million fish characterized effect on things like cytoplasm, reproductive anatomy (sperm, eggs, livebearer growth areas), brain morphology, telomere length, cardiovascular
fitness, cancer amount, and immune system effects;
One area of longevity molecule research doable at fish at a hydrosorter, is the longevizing effects of reducing the amounts of some molecules from tissues or at circulation; It is possible the hydrosorter could be used to administer gill-breathable
antigens (immunizations) against things like interleukins, sex hormones that are absent effects on secondary sex characteristics yet have cardiovascular risks or carcinogenic effects, liver enzyme saturating endogenous chemicals, mTOR activators, ILGF-1 (
and similar molecules), endogenous telomere length reducing molecules, endogenous AMPK activity decreasing chemicals, possibly some versions of cortisol-like molecules circulating endogenously, physiochemicals that get upregulated when organisms miss
sleep and could be deleterious (missing one night of sleep doubles likelihood of infection at a human); peptides (or proteins)that cause stress, possibly like deleterious versions of beneficial peptides (or proteins) that occupy beneficial receptors, but
have zero or negative activity, keeping the beneficial forms of those peptides (or proteins) from reaching the receptors and providing benefit, various lipopolysaccharides that might reach the circulatory system from the GI tract, histamine, the
physiological chemicals produced at gum disease that I read is like 20something to 48% active at causing cardiovascular disease, Any endogenous physiochemicals that cause heart rythms outside the wellness standard (although these are of course fish), as
well as, noting that a combination of epithalon with thymosin causes four times less mortality during six years at humans, any chemicals that occupy epithalon and thymosin receptors causing those receptors to not function at preserving living function.
A peltier enabled hydrosorter used at fish could also be used to develop cryogenic cryoprotectants to support human cryonic preservation and revival.
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